Laboratory

Laboratory of Biotechnology

Vision

To be a laboratory of industrial biotechnology that acts as a center of technology research and development in the food and agricultural products fields that have benefit to the society both in national and international level.

Mission

  • Increasing students` competencies in the industrial food biotechnology and agricultural products and also entrepreneurial skill
  • Developing research in the industrial food biotechnology and agricultural products by using lecturers` and students` research
  • Make cooperation with various group in terms of research and community service in the industrial food biotechnology and agricultural products fields
  • To popularize the results of research of industrial food biotechnology and agricultural products fields that are able to encourage the Indonesian economy and keep maintaining the environment

Goals

  • Support good education in order to develop science and technology in industrial food biotechnology and agricultural products fields and able to apply it to the community
  • Produce high quality research work in industrial food biotechnology and agricultural products fields that is able to encourage the development of food-based industries and agricultural products
  • Actively participate in the dissemination of science and technology in in industrial food biotechnology and agricultural products fields to add society and industry values

Motto

DO THE BEST AND BE THE BEST

Agreement To Service

  • We are here to serve You
  • Your Satisfaction is Our concern
  • We serve You with Our Heart

Service Ethics

  • Initiated by smiling and greeting
  • Always wearing of identification (identity card)
  • Be polite towards service users
  • Do not arbitrarily to service users
  • Do not justified in providing services while smoking or while communicating with mobile phones or other communication devices

Head of Laboratory

Prof. Agustin KrisnaWardani, STP. M.Si., PhD

Staff

Vita Nur Mufidah, STP


Equipment / Instrument :

# Name Qty Users /day
1 Autoclave sterilization (ES 315) 1 4
2 Autoclave destruction (Autoclave/Pressure steam sterilizer electric) 2 4
3 Incubator CO2 Compact Air - Jacketed CO2 Incubator (Automatic CO2) 1 1
4 Analytic scale 2 10
5 Fermentor 1 1
6 Gel documentation 2 10
7 PCR (Polymerase Chain Reaction) GeneAmp PCR System 9700 1 1
8 Hotplate stirer 4 8
9 Laminar Air Flow (LAF) 1 4
10 Mikropipet 100-1000 mikrolit 10 20
11 Mikropipet 10-100 mikrolit 4 8
12 Micropipette 20-200 mikrolit 2 4
13 Micropipette 1-10 microlit 2 4
14 micropipette 2-20 microlit 2 4
15 micropipette 0.1-2.5 microlit 5 10
16 Microplate Reader ELx800 1 4
17 Microscope 2 10
18 Centrifuge mini/Spindown 1 10
19 Incubator 3 6
20 pH meter 2 6
21 Microwave 1 4
22 electrophoresis protein /SDS PageVertical chamber (protein) 3 6
23 Cold Sentrifuge / Falcon 1 5
24 Spectrophotometer UV Vis 2 4
25 Vortex 3 6
26 Shaker Waterbath 2 2
27 Nanodrop (Spectrophotometric ) 1 5
28 Electrophoresis DNA/Horizontal Electrophoresis 5 10
29 Electrophoretic Transfer Cell Mini Trans-Blot® 1 2
30 Incubator shaker 3 3
31 Deep Freezer Ultra Low Temperature DF 490 1 20
32 Freezer 1 20
33 Flake ice maker 1 10
34 refrigerator 2 10
35 Biological Safety Cabinet (Cat No.: 1384 – G) 1 4
36 Centrifuge Micro 1.5 ml 1 5
37 Drybath 1 5
38 Fermentor/Bioreactor Minifors 1 1
39 Refrigerator (showcase) 1 4
40 Micropipette 0.5-10 µl 5 10
41 Micropipette 30 -300 µL 1 2
42 Micropipette 500 -5000 µL 1 2
43 Micropipette 1000 -5000 µL 5 10
44 Microplate Reader ELx800 1 5
45 Oven 1 2
46 Penutup UV Transiluminator 1 1
47 Stirer 1 2
48 UltraPure Water Purifications Systems Life Sciences 1 10

Culture Collections :

# Name
1 Lactobacillus casei FBCC 001
2 Lactobacillus plantarum FBCC 002
3 Acetobacter aceti FBCC 003
4 Bacillus natto FBCC 004
5 Bacillus amyloliquefaciens FBCC 005
6 Bacillus subtilis FBCC 006
7 Bacillus cereus FBCC 007
8 Pseudomonas aeruginosa FBCC 008
9 Shigella flexneri FBCC 009
10 Saccharomyces cerevisiae FBCC 010
11 Candida albicans FBCC 011
12 Escherichia coli FBCC 012
13 Staphylococcus aureus FBCC 013
14 Listeria monocytogenes FBCC 014
15 Escherichia coli BL21 FBCC 015
16 Escherichia coli DH5 alpha FBCC 016
17 Salmonella typhii FBCC 017
18 Shigella dysenteriae FBCC 018
19 Xanthomonas oryzae FBCC 019
20 Vibrio parahaemolyticus FBCC 020
21 Vibrio cholerae FBCC 021
22 Kluyveromyces marxianus FBCC 022
23 Lactobacillus lactis FNCC 0120
24 Lactobacillus rhamnosus FNCC 0052
25 Bifidobacterium bifidum FNCC 0462
26 Bacillus coagulans FNCC 0081
27 Salmonella typhimurium 0050 (IFO 12529)

Software :

# Name
1 Design Expert 12
2 Docking Software
3 Mendeley
4 Google Classroom
5 Benchling

Infrastructure :

# Name Qty
1 Eyewash 1
2 Fire Extinguisher 1
3 CCTV 2
4 Wireless Fidelity (WiFi) 1
5 Uninterruptible Power Supply (UPS) 4
6 First aid box 1

List Of Publication :

# Title Year Software
1

Evaluation and analysis of transglutaminase-modified gluten-free soybean bread processed using ohmic heating with varied voltage levels

A Sutrisno, JP Hutasoit, ES Murtini, FD Effendi, S Prakash

Innovative Food Science & Emerging Technologies 100, 103904

 2025 -
2

Screening Adaptive Microbial Hydrolytic Enzymes for Cleaner Production in Resource-Limited Settings

T Mahatmanto

Industrial Biotechnology

 2025 -
3

Simultaneous Extraction and Decaffeination Process Optimization of Green Coffee Bean-Based Beverages Using Response Surface Methodology

NIP Nugrahini, AK Wardani, E Martati, MS Rohman

Trends in Sciences 21 (6), 7265-7265

 2024 -
4

Investigation of the Physicochemical Properties and its Correlation during Koji-Moromi Fermentation Stage of Production Soy Sauce Naturally Brewed in Central Java, Indonesia.

BE Setiani, E Zubaidah, AK Wardani

International Journal on Advanced Science, Engineering & Information Technology 14 (2)

 2024 -
5

The Optimal Dose of the Green Tea and Coffee Extracts to Suppress the Expression of PPAR-γ and C/EBP-α on Differentiated 3T3-L1 Adipocytes

NIP Nugrahini, MS Rohman, AK Wardani, E Martati, IN Chomsy

Trends in Sciences 21 (1), 7182-7182

 2024 -
6

The effect of corn starch and transglutaminase on quality improvement of soybean-based analog rice

A Sutrisno, ANF Suloi, ES Murtini, NA Bahmid

Future Foods 10, 100407

 2024 -
7

Optimization of enzymatic ramie degumming using thermoalkaliphilic xylanase from Bacillus halodurans CM1 and a commercial pectinase

Dadang Suhendar, Muhammad Dimas Azka Maulana, Aji Sutrisno, Deden Rosid Waltam, Kharis Yohan Abidin, Dewi Nandyawati, Niknik Nurhayati, Erma Widyasti, Edi Wahjono, Dicky Adihayyu Monconegoro, Haniyya Haniyya, Catur Sriherwanto, Noorendra Laksamana Putra, Nanik Rahmani

Biocatalysis and Biotransformation 42 (6), 749-759

 2024 -
8

Exploring the Antidiabetic and Antihypertensive Potential of Peptides Derived from Bitter Melon Seed Hydrolysate

WT Hung, CCY Sutopo, T Mahatmanto, ML Wu, JL Hsu

Biomedicines 12 (11), 2452

 

 2024 -
9

In Silico Analysis of Protein of Milk, Soybean, and Kefir as Anti-Thrombotic Bioactive Peptide

MI Fahri, F Sherlyna, M Nurcholis

HAYATI Journal of Biosciences 30 (2), 216-223

 2023 PyMOL 2.5, Autodock Vina, BIOVIA Discovery Studio, Mendeley, Microsoft Word, Microsoft Excell
10

Photostability study of new photosensitizer from dregs of green tea and its antibacterial adjustment against Escherichia coli ATCC 8739. R Indrawati

AIP Conference Proceedings

 2022 Mendeley, Microsoft Word, Microsoft Excell
11

An exploration of angiotensin-converting enzyme (ACE) inhibitory peptides derived from gastrointestinal protease hydrolysate of milk using a modified bioassay-guided fractionation approach coupled with in silico analysis

Journal of Dairy Science Volume 105, Issue 3, March 2022, Pages 1913-1928

 2022 GraphPad 5, PEAKS X+, Accelrys Discovery Studio Visualizer version 3.0, Mendeley, Microsoft Word, Microsoft Excell
12

An exploration of ACE inhibitory peptides derived from gastrointestinal protease hydrolysate of milk using a modified bioassay-guided fractionation approach coupled with in silico analysis.

S Ningrum, A Sutrisno, JL Hsu - Journal of Dairy Science, 2022

 2022 GraphPad 5, PEAKS X+, Accelrys Discovery Studio Visualizer version 3.0, Mendeley, Microsoft Word, Microsoft Excell
13

Eco-friendly production of silica particles and fertilizer from rice husk, rice straw, and corncob wastes

RU Maulana, SI Yanti, RZ Makrudi, T Mahatmanto, U Murdiyatmo

Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

 2022 Mendeley, Microsoft Word, Microsoft Excell
14

Feather Waste Biorefinery using Chryseobacterium sp. A9. 9 Adapted to Feathers as its Sole Carbon and Nitrogen Source

T Mahatmanto, NR Estiningtyas, SK Maharani, AS Shalahuddin, ...

Waste and Biomass Valorization, 1-10

 2022 ABI PRISM 3730xl Genetic Analyzer, Mendeley, Microsoft Word, Microsoft Excell
15

Antioxidant Content of Tisane of Cocoa Bean Shells as Affected by Roasting Temperatures

Gumelar, H Firmanto, M Nurcholis

Pelita Perkebunan (a Coffee and Cocoa Research Journal) 38 (3), 200-210

 2022 Mendeley, Microsoft Word, Microsoft Excell
16

Ethanol Production from Oil Palm Trunk: A Combined Strategy Using an Effective Pretreatment and Simultaneous Saccharification and Cofermentation

AK Wardani, A Sutrisno, TN Faida, RD Yustina, U Murdiyatmo

International Journal of Microbiology 2021

 2021 Design Expert, Mendeley, Microsoft Word, Microsoft Excell
17

Exploring cellulolytic microorganisms from coffee industry by-products and their enzyme properties

AA Brahmanti, E Martati, AK Wardani

IOP Conference Series: Earth and Environmental Science 924 (1), 012075

 2021 Unsrambler X, MEGA 7, Mendeley, Microsoft Word, Microsoft Excell
18

Detection of Pork DNA in Meatballs and Ice Cream Using Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP)

AK Wardani, A Sutrisno, HA Prabowo, N Fendy, F Kurniawan

Enhancing Halal Sustainability, 237-247

 2021 Agilent RFLP Decoder Software (FISH), Mendeley, Microsoft Word, Microsoft Excell